Quantifying western blots: none more black

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Phenylglyoxal-based visualization of citrullinated proteins on Western blots.

Citrullination is the conversion of peptidylarginine to peptidylcitrulline, which is catalyzed by peptidylarginine deiminases. This conversion is involved in different physiological processes and is associated with several diseases, including cancer and rheumatoid arthritis. A common method to detect citrullinated proteins relies on anti-modified citrulline antibodies directed to a specific che...

متن کامل

When are Two Rewrite Systems More than None?

It is important for programs to have modular correctness properties. We look at non-deterministic programs expressed as termrewriting systems (which compute normal forms of input terms) and consider the case where individual systems share constructors, but not de ned symbols. We present some old and new su cient conditions under which termination (existence of normal forms, regardless of comput...

متن کامل

Imaging of Chemiluminescent Western Blots: Comparison of Digital Imaging and X-ray Film

The decay of signal over time was performed using serial dilution of a E. coli cell lysate containing a 51 kD polyhistidinetagged recombinant protein run on a 4–20% linear gradient Criterion gel. Blots were probed with mouse anti-polyhistidinetag primary antibody followed by incubation with GAM-HRP secondary antibody and StrepTactin HRP (1:10,000) for 1 hr each. The blots were then cut into two...

متن کامل

Multiplex detection and quantitation of proteins on western blots using fluorescent probes.

The uses of multiplex detection methodologies are dramatically increasing as a means to increase sample throughput and to demonstrate quantitative differences between multiple targets in gene or protein expression analysis. In this study, we investigate the application of multiplex fluorescent detection for three proteins on the same Western blot using a laser-scanning imaging system, the Bio-R...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: BMC Biology

سال: 2016

ISSN: 1741-7007

DOI: 10.1186/s12915-016-0339-1